Figure 1
From: Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris
Characterization of putative genes involved in L-rhamnose metabolism in P. pastoris.
(A) Growth profiles of P. pastoris grown on 2% (w/v) L-rhamnose or glucose as a sole carbon and energy source. (B) Relative positions of putative genes involved in L-rhamnose metabolism in the chromosome of P. pastoris. LRA1, L-rhamnose 1-dehydrogenase; LRA2, L-rhamnono-ɤ-lactonase; LRA3, L-rhamnonate dehydratase; LRA4, L-KDR aldolase. (C,D) Relative transcription profiles of these genes in P. pastoris grown in different media. The relative expression value of each gene in BMDY was assigned as 1 and the GAPDH gene was used as a reference (C); the relative expression value of the GAPDH gene was used as a reference and was assigned as 100% (D). Each test was conducted in triplicate and the results are presented as means ± SEM of three replicates (n = 3).
