Figure 2
From: Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris
Physiological function assay of LRA4.
(A) Multiple amino acid sequence alignment of L-KDR aldolases from different strains. Ppa, Pichia pastoris; Sst, Scheffersomyces stipitis; Dha, Debaryomyces hansenii; Gll, Glarea lozoyensis; Cte, Candida tenuis. The two catalytic residues (a lysine residue and a tyrosine residue) in L-KDR aldolase are indicated with arrows. (B) Growth profiles of the wild-type strain, LRA4-disrupted strain and LRA4-complemented strain grown on glucose (left) or rhamnose (right) as a sole carbon source. (a) Wild-type strain, (b) LRA4-disrupted strain, (c) LRA4-complemented strain.
