Figure 5
From: Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris
Identification of the LRA3 minimal promoter.
(A) Promoter activities of all 5′-deletion promoter constructs were evaluated according to β-galactosidase activity in culture supernatant of recombinant strains grown in BMRY. Numbers refer to the number of nucleotides remaining in the 5′-flanking region upstream of the ATG of the initiating methionine of LRA3. (B) β-galactosidase activity and (C) β-galactosidase production in culture supernatant of recombinant strains harboring the indicated promoter to drive lacB expression at different induction time. (D) The nucleotide sequence of putative PLRA3 and the region between −140 to −210 bp (underlined). Each test was conducted in triplicate and the results are presented as means.
