Figure 1
hSSB1 oligomerizes following oxidative stress.
(a) hSSB1 oligomerizes following oxidative stress in human cells. Immunoblots of lysates from U2OS cells left untreated or treated with 250 μM H2O2 or 30 mM KBrO3 for 30 min. Lysates were resolved on a gel using non-reducing or reducing loading buffer (+/− DTT). Immunoblots were probed for endogenous hSSB1. (b) hSSB1 can self-associate. FLAG-tagged proteins were immunoprecipitated from untreated HeLa cells co-expressing 3x FLAG-hSSB1 and Myc-hSSB1 and analyzed by immunoblotting with the indicated antibodies. *3x FLAG-hSSB1, <=Myc-hSSB1. (c) Immunoprecipitation of Myc-tagged proteins from untreated HeLa cells co-expressing 3x FLAG-hSSB1 and Myc-hSSB1. Samples were analyzed by immunoblotting with the indicated antibodies. *3x FLAG-hSSB1, <=Myc-hSSB1.
