Table 3 Rate constants calculated from kinetic data in Table 1.

^aRate constant numbering is according to Fig. 1. The value of [O₂] was 250 μM.
^bk₃ is the FMN reduction rate constant k_red (Table 1).
^ck₅ was determined from its relationship with the apparent k_cat, derived from Equation 1: k₅ = k_{cat_app}/(1-k_{cat_app}/k₃-k_{cat_app}/k₇[O₂]). k_{cat_app} is for reaction at [O₂] = 250 μM.
^dk₅ is the rate constant for the second (slow) phase of absorbance decrease at 455 nm. See k_slow in Table 1.
^eNote that k₇ corresponds to k_ox (Table 1).
^fPercent rate limitation in k_{cat_app} at 250 μM O₂ due to k₅.
^gAs shown in earlier studies^1,12,13, the kinetic constants can be used to calculated the k_cat at saturating oxygen concentration and also the K_m for oxygen: k_cat = k₃k₅/(k₃ + k₅); K_m (O₂) = k_cat/k₇.

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