Figure 4: CDH2, not CDH1, blocking antibody treatment prevents porcine NP cells from forming cell clusters on soft PEG-LM, along with associated loss of juvenile NP cell phenotype features. | Scientific Reports

Figure 4: CDH2, not CDH1, blocking antibody treatment prevents porcine NP cells from forming cell clusters on soft PEG-LM, along with associated loss of juvenile NP cell phenotype features.

From: N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions

Figure 4

(A,B) Representative immunostaining of phalloidin, CDH2, and CDH1 for NP and AF cells, respectively (green = protein, red = cell nuclei, scale bar = 50 μm). (C) Changes in sGAG production for porcine NP cells on soft and stiff PEG-LM after blocking antibody treatment. (D) Quantification of gene expression for juvenile NP cell phenotype markers in porcine NP cells on soft, relative to NP cells on stiff after blocking antibody treatment (2-way ANOVA with Tukey’s post-hoc analysis, *p < 0.05) (Key: 1 = No treatment condition, 2 = CDH2 blocking antibody treatment, 3 = CDH1 blocking antibody treatment; CDH2 = N-cadherin, T = brachyury, LM1 = Laminin1, AGC = aggrecan, COL2 = type II collagen).

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