Figure 1 | Scientific Reports

Figure 1

From: CD4 T cell epitope specificity determines follicular versus non-follicular helper differentiation in the polyclonal response to influenza infection or vaccination

Figure 1

Tfh and NonTfh cells exhibit prototypical phenotype and kinetics following influenza infection.

(a) Example of sorting strategy for isolating antigen-experienced Tfh and NonTfh cells after infection. SJL mice were infected intranasally with 50,000EID50 of A/New Caledonia/20/1999. Lymphocytes were isolated from the draining mediastinal lymph node on day 9 post infection and FACS sorted based upon expression of the following markers: CD4 + CD44hiCXCR5 + PD-1 + (Tfh) or CD4 + CD44hiCXCR5negPD-1neg (NonTfh). (b,c) Kinetics of effector CD4 T cell and B cell expansion following infection depicting Tfh and NonTfh populations, as well as GC (CD19 + B220 + GL-7 + CD95+) and plasmablasts (CD19 + B220intCD138+) (n = 2–5 mice per timepoint, representative of 2 experiments). (d) Expression of Bcl6 and (e) CCR7 in Tfh (Filled Grey) and NonTfh (Black Open) populations. (f) RT-PCR analysis of total RNA from Tfh (Filled) and NonTfh (Open) showing relative expression of various transcripts isolated from the indicated sorted T cell populations, normalized to B2m expression. Data shown is from a single experiment, representative of four.

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