Figure 4: Co-localization of the fluorescence signal from the commercial lysotracker and the Resonance Raman signal from the BBQ650-Lyso probe.
From: Resonance Raman Probes for Organelle-Specific Labeling in Live Cells

The cell was simultaneously stained with the BBQ650-Lyso probe and the commercial lysotracker. Both types of lysosome probes are accumulated in the cytoplasm, and co-localize in the lysosomes, while the nucleus was not stained. Signals from (a) commercial fluorescent lysotracker and (b) BBQ650-Lyso probe. (c) The yellow color on the right panel represents the overlap of the two signals. White bar in (c) corresponds to 10 μm. The scanning rate is 4 μs/pixel (full image time scanning ~4 s).