Figure 7: CTMP regulates PI3K-mediated Akt phosphorylation through an epithelial growth factor receptor (EGFR)-independent pathway. | Scientific Reports

Figure 7: CTMP regulates PI3K-mediated Akt phosphorylation through an epithelial growth factor receptor (EGFR)-independent pathway.

From: Carboxyl-Terminal Modulator Protein Positively Acts as an Oncogenic Driver in Head and Neck Squamous Cell Carcinoma via Regulating Akt phosphorylation

Figure 7

(A–D) Each cell line was transiently transfected with CTMP-specific cDNA (A,C) or siRNA (B,D) and the expression of Akt, p-Akt at Ser 473, GSK-3β, and p-GSK-3β at Ser 9 were analyzed through Western blot analysis. (E,F) The cell lysates from each cell line were co-immunoprecipitated with an anti-Akt antibody, and the immunoprecipitation product was analyzed through Western blot for CTMP and Akt. (G) To determine the sequential relationship between Akt and CTMP, the expression of Akt, p-Akt at Ser 473, and CTMP were examined after LY294002 (15 μm for 24 h) treatment by Western blotting. (H) To elucidate the relationship between EGFR and CTMP signal pathways, the expression of Akt, p-Akt at Ser 473, and CTMP were examined after EGF and/or LY294002 treatment by Western blotting. EGF treatment additively promoted Akt phosphorylation compared to controls without altering CTMP expression.

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