Figure 3

Perampanel administration for 90 days rescued AHCs from death and normalized TDP-43 subcellular localization.

(a) Number of AHCs in L5. V, vehicle (methyl cellulose)-treated AR2 mice (n = 7); P, perampanel-treated AR2 mice (n = 8). Three sections were measured for each mouse. (b) Frequency histogram of AHCs in the perampanel group (P, blue columns) and the vehicle group (V, white columns). The vertical axis indicates the proportion of the total number of AHCs with a diameter within each range. (c) Immunostaining of the lumbar spinal cord for TDP-43 (green). White dotted lines indicate the margin of the ventral gray matter. Asterisk: TDP-43-positive cytoplasmic aggregates. TO-PRO-3 (blue) was used as a cell body marker. The scale bar indicates 100 μm (upper panels) or 20 μm (lower panels). (d) Number of TDP-43-positive (black columns) and TDP-43-negative (white columns) AHCs. Hatched columns indicate the results on vehicle-treated mice. ***p < 0.0001 against the vehicle (V) group, Wilcoxon rank sum test. (e) The number of AHCs showing TDP-43 immunoreactivity in the nucleus (N), cytoplasm (C) and both the nucleus and cytoplasm (N/C) are indicated. Hatched columns indicate the results on vehicle-treated mice. (a,b,d,e) All error bars represent the s.e.m.; *p < 0.05, **p < 0.001 and ***p < 0.0001 against the vehicle (V) group, Wilcoxon rank sum test.