Figure 1
From: Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism
Effects of LNT on S-180 tumor cells apoptosis and proliferation in vivo.
(A) Effect of LNT on S-180 tumor weight in mice, **p < 0.001 versus the control, *p < 0.05 versus cytoxan. All data in vivo are expressed as means ± SD of three independent experiments and each performed in duplicate. The data in vivo are expressed as means ± SD of 10 mice in each group. (B) Histological analysis by hematoxylin and eosin staining (H&E) of sections of tumors and spleens from mice and TUNEL staining of tumors. Scale bars, 25 μm. (C) Immunohistochemical staining for apoptotic cells (Bax and Bcl-2 staining) and proliferating cells (Ki67 staining) in tumor tissue with positive staining for the biomarker shown. Scale bars, 50 μm. (D,E) S-180 tumor tissue proteins were extracted and p53, p21, Bax, Bcl-2, caspase 3, caspase 9 and PARP1 were detected by western blot analysis using their specific antibodies with β-actin as the loading control. The blots are run under the same experimental conditions. Experiments were performed at least twice and protein expression was determined by quantitative densitometry. The results shown are representative of three independent experiments. *p < 0.05 and **p < 0.001 versus the control.
