Figure 2
From: Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism
LNT induces infiltration of immune cells and cytokines/chemokines production in S-180 tumor tissues.
(A) Immunohistochemistry of tumor tissue, stained with antibody against CD3 and CD68 (brown). Scale bars, 25 μm. (B) Quantification (IOD) of CD3 and CD68 per 100 mm2 of (A), *p < 0.05 and **p < 0.001 versus the control or cytoxan. (C) Cytokines in S-180 tumor tissue lysates by mouse cytokines array. *p < 0.05 and **p < 0.001 versus the control. (D) TGF-β in tumor lysates was assayed by ELISA, **p < 0.001 versus the control. (E) TAK1 expression in S-180 tumor tissues was detected by western blot using its specific antibody. The blots are run under the same experimental conditions. The results shown are representative of three independent experiments, **p < 0.001 versus the control.
