Figure 4
From: Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism
LNT inhibits angiogenesis in S-180 tumors.
(A) Immunohistochemical analysis of CD31 and vascular endothelial growth factor (VEGF) in S-180 tumors. Scale bars, 25 μm. (B,D) Quantification of MVD as mean number of vessels per 100 mm2, *p < 0.05 and **p < 0.001 versus the control. (C) Immunofluorescence assay of CD31. (E) Western blot analysis of tumor protein extracts to detect VEGF using its specific antibody. The blots are run under the same experimental conditions. (F) The expression of VEGF was determined by quantitative densitometry. *p < 0.05 versus the control. The images and western blot results shown are representative of three independent experiments.
