Figure 5
From: Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism
LNT suppresses Stat 3 activation in S-180 tumor tissues.
Immunohistochemical analysis (A) and its quantification of IOD per 100 mm2 (B) of p-Stat 3 in tumors. Scale bars, 25 μm. *p < 0.05, **p < 0.001 versus the control. Immunofluorescence assay (C) and its quantification of IOD per 100 mm2 (D) of p-Stat 3. Scale bars, 25 μm. (E) Western blot analysis of phospho-Stat 3 and total Stat 3 in protein extracts prepared from tumors (representative of three experiments) using their specific antibodies. The blots are run under the same experimental conditions. (F) The digital results of Stat 3 and p-Stat 3 were determined by quantitative densitometry. *p < 0.05 versus the control. The image and western blot results shown are representative of three independent experiments.
