Figure 6

Effect of LNT on cell viability and apoptosis in Hela and S-180 tumor cells.

(A) Cell viability of H8, LO2 and 293T cells after treatment with LNT for 48 h determined by MTT assay. (B) Cell viability of Hela and S-180 cells after treatment with LNT for 48 h determined by MTT assay. *p < 0.05 and **p < 0.001 versus the control (0 μg/mL). Hela (C) and S-180 (E) cells were respectively treated with LNT (200 μg/mL, 400 μg/mL) for indicated time intervals and the whole cell extracts were prepared, 30 μg of which were resolved on 10% or 12% SDS-PAGE. p53, p21, Bax, Bcl-2 and caspase 3 were detected by western blot analysis using their specific antibodies with β-actin as the loading control. (D,F) The digital results were determined by quantitative densitometry. The results shown are representative of three independent experiments. *p < 0.05 and **p < 0.001 versus the control. (G) Hela cells were transfected with p53 siRNA and then treated with LNT (200 μg/mL) for indicated time intervals and the whole cell extracts were prepared, 30 μg of which were resolved on 10% or 12% SDS-PAGE. p53, p21, Bax, Bcl-2 and caspase 3 were detected by western blot analysis using their specific antibodies with β-actin as the loading control. The blots are run under the same experimental conditions. (H) Hela cells were transfected with p53 siRNA and then treated with or without LNT at desired concentration for 48 h. Cell viability was determined by MTT assay.