Figure 4 | Scientific Reports

Figure 4

From: Loss of cortactin causes endothelial barrier dysfunction via disturbed adrenomedullin secretion and actomyosin contractility

Figure 4

Adrenomedullin is secreted to a lesser extent without cortactin.

(A) Confluent HUVEC monolayers were cultured for 3 days in fresh medium before collection of supernatants and analysis of ADM concentrations by ELISA. (B) Blood from WT and cortactin KO mice was extracted from the retro-orbital plexus. Plasma was analysed by ELISA to determine ADM concentrations. Graphs show means +/−SDM of three independent cell cultivations or 6 mice, respectively. (C) cDNA was analysed by real-time RT-PCR for expression of ADM. Data are normalized to β-actin as house-keeping gene.**p < 0.01; ***p < 0.001.

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