Figure 1: Schematic of the hph and eGFP/mRFP cassettes made in this study. | Scientific Reports

Figure 1: Schematic of the hph and eGFP/mRFP cassettes made in this study.

From: A native promoter and inclusion of an intron is necessary for efficient expression of GFP or mRFP in Armillaria mellea

Figure 1

The hph cassette was amplified from pBGgHg and has the Agaricus bisporus gpdII promoter driving hph with the CaMV 35S terminator30. Armillaria mellea (Am) long and short introns are introns 11 and 7, respectively, from putative efflux transporter genes (NCBI reference EF547153 and EF547152, respectively)48. The Phanerochaete chrysosporium gpd (Pc gpd) promoter, P. chrysosporium intron/exon, eGFP and the Aspergillus nidulans trpC terminator (An trpC) were amplified from pGR4-GFP or pGR4-4iGM323. mRFP was amplified from pYES-hph-RFP00424. The sequence of the A. mellea gpd promoter (Am gpd) and intron was based on the gene model with protein ID 1312513.

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