Table 1 Armillaria mellea and Clitopilus passeckerianus transformants obtained with various vectors and the percentage showing expression of GFP or mRFP.
Vector | Promoter | Intron | Number of C. passeckerianus transformants (% expressing GFP/mRFP) | Number of A. mellea transformants (% expressing GFP/mRFP) |
|---|---|---|---|---|
pBGgHg30 | Agaricus bisporus gpdII | — | 29 (0%) | 31 (0%) |
pGR4-4iGM323 | A. bisporus gpdII | P. chrysosporium gpd | n/a | 27 (0%) |
pGR4-GFP23 | Phanerochaete chrysosporium gpd | P. chrysosporium gpd | n/a | 23 (0%) |
pCAM-hph-GFP | P. chrysosporium gpd | — | 6 (0%) | 10 (0%) |
pCAM-hph-mRFP | P. chrysosporium gpd | — | 11 (0%) | 23 (0%) |
pCAM-hph-Amgpd-GFP | Armillaria mellea gpd | — | 5 (0%) | 10 (0%) |
pCAM-hph-Amgpd-mRFP | A. mellea gpd | — | 13 (0%) | 11 (0%) |
pCAM-hph-LiGFP | P. chrysosporium gpd | A. mellea et a | 6 (0%) | 10 (0%) |
pCAM-hph-siGFP | P. chrysosporium gpd | A. mellea et b | 4 (100%) | 17 (0%) |
pCAM-hph-simRFP | P. chrysosporium gpd | A. mellea et b † | 6 (0%) | 11 (0%) |
pCAM-hph-iGFP | P. chrysosporium gpd | A. mellea gpd | 5 (40%) | 10 (0%) |
pCAM-hph-imRFP | P. chrysosporium gpd | A. mellea gpd | 3 (33%) | 16 (0%) |
pCAM-hph-xiGFP | P. chrysosporium gpd | P. chrysosporium gpd | 26 (96%) | 18 (0%) |
pCAM-hph-Amgpd-iGFP | A. mellea gpd | A. mellea gpd | 4 (100%) | 10 (20%) |
pCAM-hph-Amgpd-imRFP | A. mellea gpd | A. mellea gpd | 1 (100%) | 4 (75%) |
pCAM-hph-Amgpd-xiGFP | A. mellea gpd | P. chrysosporium gpd | 5 (60%) | 10 (30%) |
pCAM-hph-Amgpd-ximRFP | A. mellea gpd | P. chrysosporium gpd | 4 (75%) | 6 (50%) |
