Figure 1 | Scientific Reports

Figure 1

From: Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification

Figure 1

Verification and optimization of hairpin (HP) cleavage-ligation.

(A) Schematic illustration of dumbbell probe (DP) formation by cleavage-ligation of hairpin. (B) DP formation analyzed by PAGE. Only after cleavage, hairpin can be ligated into a band-shifted DP, which can be recleaved into hairpin. (C) All of the three kinds of cleaved ends produced by EcoRI, PstI and EcoRV are suitable to form DP efficiently. (D) Effects of stem length at the end side of the RE recognition sequence on DP formation. NsiI recognition sequence is underlined. (E) Effects of stem length at the loop side of the RE recognition sequence on DP formation. EcoRI recognition sequence is underlined.

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