Figure 6
Enhanced specificity by two-ligation design and the 2nd amplification.
(A) Schematic of the two ligation points: arrow represents the left ligation point and triangle represents the right ligation point. (B) Let-7 family sequences alignment marked with ligation points. (C~J) Family members’ discrimination by RCA. Single left ligation point for discriminating let-7a by fluorescence signal (C) and relative RCA rate (G); single right ligation point for discrimination by fluorescence signal (D) and relative RCA rate (H); two ligation points for discrimination by the single RCA fluorescence signal (E) and relative RCA rate (I); two ligation points for discrimination by the C2C RCA fluorescence signal (F) and relative RCA rate (J). Relative RCA rates (RRR) in (G~J) were calculated by the slope of fluorescence curve in (C~F).
