Figure 2: SIRT1 transgenic mice exert a protective effect on alcohol-induced hepatic steatosis.

(a) H&E staining performed on liver sections from mice. (b) Body weight, liver weight, hepatic TG, and cholesterol in wild type and SIRT1 transgenic mice. (c) Western blot analysis of SIRT1, PPARγ, and β-actin. (n = 5 per group) (d) PPARγ acetylation in wild type and SIRT1 transgenic mice. (n = 3 per group) (e) Real-time PCR analysis of PPARγ and its target genes in liver. (f) AST and ALT levels in blood samples. (n = 6 per group). Data represent the mean ± SD. *P < 0.05, **P < 0.01.