Figure 5: MGAT1 knockdown suppresses alcohol-induced hepatic steatosis. | Scientific Reports

Figure 5: MGAT1 knockdown suppresses alcohol-induced hepatic steatosis.

From: Suppression of PPARγ-mediated monoacylglycerol O-acyltransferase 1 expression ameliorates alcoholic hepatic steatosis

Figure 5

(a) Schedule of MGAT1 knockdown in ethanol-fed mice. Adenoviral sh-control or sh-MGAT1 was introduced via tail vein injection at 3 weeks after initiation of ethanol feeding. One week later, the mice were sacrificed. (b) H&E staining performed on liver sections from mice in paired, ethanol-fed with sh-control, and ethanol-fed with sh-MGAT1 groups. (c) Body weight, liver weight, hepatic TG, and cholesterol content. (d) Real-time PCR analysis showing the expression of PPARγ and its target genes. (n = 6). Data represent the mean ± SD. *P < 0.05. (e) Primary hepatocytes (4 × 105 cells per well) cultured on 6-well plates were infected with 50 MOI of Ad-GFP, Ad-PPARγ2, and Ad-SIRT1 with or without nicotinamide (NA, 10 μM). MGAT1 mRNA was analyzed by real-time PCR. (f) Luciferase assay using human MGAT1 promoters (~2 kb) was performed. In HepG2 cells, MGAT1 promoter constructs were co-transfected with or without PPARγ2/RXRα overexpression vectors along with 100 MOI of either Ad-GFP or Ad-SIRT1. Data represent the mean ± SD from three independent experiments performed in duplicate. *P < 0.05, **P < 0.01.

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