Figure 7
DSP aa183-219 up-regulates DSPP gene transcription through SMAD1/5/8.
(A) pGL-Luc-mDSPP-241/+54 (p241) were co-transfected with 100 ng of pcDNA-Smad4, pCMV-Smad1/5/8, or pcDNA3.1. The transcription results were computed as luciferase activities per mg of total protein. The value obtained from the control group was considered as 1-fold. Fold increases were calculated by dividing the individual value by the control group. (B) p241 Wt, p241 Mut-1, p241 Mut-2, or p241 Mut-3 were co-transfected with 100 ng pcDNA-Smad4. (C) p241 Wt, p241 Mut-1, p241 Mut-2, or p241 Mut-3 were co-transfected with 100 ng pcDNA-Smad1/5/8. (D) p241 Wt, p241 Mut-1, p241 Mut-2, or p241 Mut-3 were co-transfected with 100 ng pcDNA-Smad4 and 20 ng Smad1/5/8. The data are the mean ± S.D. from independent experiments performed in triplicate.
