Table 1 L-DOPA production in different E. coli strains*.
From: Genome engineering Escherichia coli for L-DOPA overproduction from glucose
Strain | Genetic modification of the host strain | OD600 | Tyrosine (mg/L) | L-DOPA (mg/L) |
|---|---|---|---|---|
E. coli BW25113 (pQE-hpaBC) | 5.55 ± 0.08 | 292.5 ± 5.2 | 138.7 ± 4.9 | |
E. coli AROM-1 (pQE-hpaBC) | E. coli BW25113, ∆tyrR, ∆csrA | 6.13 ± 0.06 | 263.5 ± 60.8 | 148.3 ± 11.7 |
E. coli AROM-2 (pQE-hpaBC) | E. coli BW25113, ∆tyrR, ∆csrA, ∆ptsHI, ∆crr | 4.57 ± 0.04 | 366.2 ± 11.8 | 176.0 ± 3.6 |
E. coli AROM-3 (pQE-hpaBC) | E. coli BW25113, ∆tyrR, ∆csrA, ∆ptsHI, ∆crr, P37-galP-P37-glk | 4.56 ± 0.07 | 304.0 ± 25.5 | 173.9 ± 11.7 |
E. coli AROM-4 (pQE-hpaBC) | E. coli BW25113, ∆tyrR, ∆csrA, ∆ptsHI, ∆crr, P37-galP-P37-glk, ∆zwf | 4.50 ± 0.03 | 256.9 ± 7.8 | 205.3 ± 2.5 |
E. coli TYR-1 (pQE-hpaBC) | E. coli BW25113, ∆tyrR, ∆csrA, ∆ptsHI, ∆crr, P37-galP-P37-glk, ∆zwf, ∆pheLA | 4.59 ± 0.05 | 256.6 ± 4.8 | 209.2 ± 0.9 |
E. coli DOPA-1 | E. coli TYR-1, attPP21::7P37-tyrAfbr-tyrB-hpaBC fusion protein chimera | 4.24 ± 0.09 | 241.3 ± 6.2 | 307.4 ± 3.7 |
