Table 5 Strains and plasmid used in this study.
From: Genome engineering Escherichia coli for L-DOPA overproduction from glucose
Strains/Plasmids | Reference | |
|---|---|---|
Strain | ||
E. coli BW25113 | lacI q rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78 | |
E. coli AROM-1 | E. coli BW25113, ∆tyrR, ∆csrA | This study |
E. coli AROM-2 | E. coli AROM-1, ∆ptsHI, ∆crr | This study |
E. coli AROM-3 | E. coli AROM-2, P37-galP-P37-glk | This study |
E. coli AROM-4 | E. coli AROM-3, ∆zwf | This study |
E. coli TYR | E. coli AROM-4, ∆pheLA | This study |
E. coli DOPA-1 | L-DOPA producer, E. coli TYR derivative integrated the tyrAfbr, tyrB and hpaBC fusion protein chimera under the control of 7P37 promoter | This study |
E. coli DOPA-2 | E. coli DOPA-1, ∆mutS | This study |
E. coli DOPA-30 | MAGE strain with the artificial 5′-UTR sequence of the hpaC | This study |
E. coli DOPA-30N | MAGE strain with the native 5′-UTR sequence of the hpaC | This study |
Plasmid | ||
pQE30 | Expression vector, T5 promoter, pBR322 ori, Ampr | Invitrogen |
pQE30-hpaBC | pQE30 containing E. coli W hpaBC | This study |
pQE30-2hpaBC | pQE30 containing 2 copies of E. coli W hpaBC | This study |
pOSIP-CH | Integration vector, HK022 integrase, attPHK022 aite, ccdB gene, catr | |
pXMJ19 | C. glutamicum-E. coli shuttle expression vector, Ptac, IPTG inducible, catr; GenBank No. AJ133195 | |
pK-JL | pK18mobsacB derivative, sacB under the control of the tac-M promoter, Kanr | |
pMD-ccdBKanS | ccdB-kan-IsceI cassette | This study |
pBAD30 | Expression vector, PBAD promoter, arabinose induction, pACYC184 ori, Ampr | |
pBAD30-I-SceI | pBAD30 derivate with the I-SceI endonuclease gene | This study |
pSIM6 | pSC101 repliconts PL-gam-bet-exo cI857, Ampr | |
pSIMIS | pSIM6 derivative with the arabinose-inducible I-SceI endonuclease gene | This study |
pZSBP | Biobrick vector, P37 promoter, pSC101 ori, Kanr | |
pZSBP-P37-glk | pZSBP derivative with the glk under the control of the P37 promoter, respectively | This study |
pZSnP37 | pZSBP derivative with the nP37 promoter (n = 2,3,4,5,6, or 7) | This study |
pZSBP-P37-galP | pZSBP derivative with the galP under the control of the P37 promoter | This study |
pHKKT5b | Integration expression plasmid, attPHK site, PT5 promoter, Kanr | |
pHKKT5b-P37-glk | pHKKT5b derivative with the glk under the control of the P37 promoter | This study |
pHKKT5b-P37-galP-P37-glk | pHKKT5b derivative with the galP and glk under the control of the P37 promoter, respectively | This study |
pP21KT5b | Integration expression plasmid, attPP21 site, PT5 promoter, Kanr | |
pP21KT5b-7P37-tyrAfbr-tyrB-hpaBC | pP21KT5b derivative with the tyrAfbr-tyrB-hpaBC fusion chimera under the control of the 7P37 promoter | This study |
pCas | repA101(Ts) ori, kanr, Pcas-cas9, ParaB-Red, lacIq, Ptrc-sgRNA-pMB1 | |
pTargetF | sgRNA plasmid, pMB1 ori, Sper | |
pTagetF-hpaC | sgRNA-hpaC plasmid, pMB1 ori, Sper | |
