Figure 2: Duodenal digestion of wheat LTP. | Scientific Reports

Figure 2: Duodenal digestion of wheat LTP.

From: Ligand binding to an Allergenic Lipid Transfer Protein Enhances Conformational Flexibility resulting in an Increase in Susceptibility to Gastroduodenal Proteolysis

Figure 2

(A,B) SDS PAGE analysis of digestion under reducing conditions at various time points in the absence (A) or presence (B) of 0.26 mM linoleic acid (LA); lane R is a reference showing wheat LTP following the duodenal digestion procedure but in the absence of trypsin and chymotrypsin. (C) MALDI-ToF MS spectra of the duodenal digests of wheat LTP after 120 min in the absence (red spectra) or presence (blue) of 0.26 mM linoleic acid. The peptides were relativity quantified by comparing spectral intensities of the same peptide in the absence or presence of linoleic acid. (D) Densitometric analysis of SDS PAGE shown in (A,B).

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