Figure 2
From: Reduced insulin/insulin-like growth factor signaling decreases translation in Drosophila and mice
Protein synthesis is reduced in the muscles of Irs1 mutants, without decreased polysome formation.
(a) Representative polysome profiles of dissected mouse tissues (74 week old). Insets show area under the curve measurements for monosomes and combined polysomes, wt (n = 4), Irs1−/− (n = 3). Note that the amount of material loaded varied per tissue, so profiles should not be directly compared between tissues. Unpaired student t-test was used to establish significance. (b) Relative 35S counts in Irs1−/− mouse tissues, wt (n = 4), Irs1−/− (n = 3). For all conditions n = 4 unless stated otherwise. Unpaired student t-test was used to establish significance. *p < 0.05.
