Figure 3
From: Reduced insulin/insulin-like growth factor signaling decreases translation in Drosophila and mice
Translation is non-tissue autonomously reduced upon loss of Irs1.
(a) Representative polysome profiles of dissected mouse tissues from muscle specific (Ckmm-Cre::Irs1fl/fl) and liver specific (Alfp-Cre::Irs1fl/fl) Irs1 knockouts compared to controls (Irs1fl/fl) (n ≥ 3). Insets show area under the curve measurements for monosomes and combined polysomes. Unpaired student t-test was used to establish significance. (b) 35S counts for muscle specific (Ckmm-Cre::Irs1fl/fl) Irs1 knockouts and controls (Irs1fl/fl). (c) 35S counts for liver specific (Alfp-Cre::Irs1fl/fl) Irs1 knockouts and controls (Irs1fl/fl). Values were first normalized to protein content, then to batch average. (d) 35S counts in an independent set of Alfp-Cre::Irs1fl/fl mice to verify the findings of Fig. 3C. For all conditions n = 4 unless stated otherwise. Unpaired student t-test was used to establish significance. *p < 0.05, **p < 0.01, ***p < 0.001.
