Figure 1 | Scientific Reports

Figure 1

From: Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion

Figure 1

Schematic for the initiation of O-linked, lipid linked and N-linked glycosylation.

(a) Core-1 derived O-glycan biosynthesis begins with the formation of the T-antigen using the T-synthase C1GalT1 and its chaperone COSMC. (b) UGCG adds the first Glc to ceramide to form Glc-Cer glycolipids. (c) MGAT1 catalyzes the addition of GlcNAc to the Man-5 substrate. Knocking out these three key enzymes results in premature termination of specific types of glycoconjugates. All figures use the Consortium for Functional Glycomics symbolic nomenclature (shown in legends).

Back to article page