Figure 2: CLI1s are activated just before motoneurons during forward motor waves.
(a) CLI1-Gal4 (tsh-Gal80; R47E12-Gal4, Cha-Gal80) drives expression specifically in a pair of CLI1s in each abdominal segment (as visualized with UAS-mCD8GFP). (b) Calcium imaging of CLI1s and aCC. Maximum intensity projection of imaged movies showing ROIs (left), and the traces of fluorescence change in each ROI (right) are shown. (c) CLI1s are activated just prior to aCCs in the same segment. Time difference between the activities of CLI1 and aCC in the same segment is plotted against the intersegmental time delay (time difference between the activities of aCCs and those in the next anterior segment). A3, 27 waves from 6 samples; A4, 27 waves from 6 samples; r: Pearson’s correlation coefficient. (d,e) Time difference between CLI1s in neighboring segments (d, 71 waves from 15 samples) or that between CLI1s and aCCs in the next anterior segment (e, 68 waves from 15 samples) is proportional to the intersegmental time delay. (f) Phase representation of the activity timing of CLI1. Activity timing of CLI1s in each segment [An; n = 1–4] normalized with that of aCCs in the same [An] and next posterior [An + 1] segment set as 100 and 0%, respectively. Activity timings are represented in box-and-whisker plots. Average phases are indicated above the boxes. Wave numbers (n) and sample numbers (N) are indicated in the parenthesis. The experiments were done using wandering third instar larvae.
