Figure 2 | Scientific Reports

Figure 2

From: ZCF32, a fungus specific Zn(II)2 Cys6 transcription factor, is a repressor of the biofilm development in the human pathogen Candida albicans

Figure 2The alternative text for this image may have been generated using AI.

The absence of ZCF32 results in an enhanced biofilm formation.

(A) The visual appearance of biofilms developed in 12 well plate using Spider medium till 60 h. Biofilm formed by YPK102 appeared denser than SC5314 biofilm. (B) SC5314, YPK101, YPK102 and YPK103 strains were grown in biofilm mode in 12-well plates for 60 h and quantitation of total biofilm was carried out by the crystal violet staining assay. The experiment was carried out three times independently and results are plotted as the standard error of mean (SEM) using Graph Pad Prism5 (*p-value ≤ 0.02). Statistical significance was calculated using one-way analysis of variance (ANOVA) coupled with Bonferroni’s test. (C) Biofilm biomass determination was done by the dry mass measurement for SC5314, YPK101, YPK102 and YPK103. Strains were grown in standard biofilm conditions for 60 h. Results from three sets of independent experiments were considered and statistical significance was calculated using one-way ANOVA coupled with Bonferroni’s post test. Results are shown as SEM (**p-value ≤ 0.02).

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