Figure 3: The majority of HHLA2 expression is on the tumor.

(A) OS1 tumor demonstrated HHLA2 expression in a small area of the tumor. CD45 positive myeloid cells, which were clearly HHLA2 negative, were present. (B) OS2 tumor demonstrated HHLA2 expression homogenously throughout the tumor. CD45 positive myeloid cells, some of which were HHLA2 positive, were present. (C) Kidney tissue demonstrated HHLA2 expression in the renal tubules. Of the CD45 positive myeloid cells present, a small fraction were also HHLA2 positive. (D) Brain tissue was negative for both HHLA2 expression and CD45 myeloid cell infiltration.