Figure 2 | Scientific Reports

Figure 2

From: Effects of T592 phosphomimetic mutations on tetramer stability and dNTPase activity of SAMHD1 can not explain the retroviral restriction defect

Figure 2

Tetramerization of SAMHD1115-626 D311A and D311A/T592D as a function of nucleotide concentration.

Model-free van Holde – Weischet analysis of the sedimentation velocity experiments for the dATP (A–C) and GTP (D–F) titration series performed on SAMHD1115-626 D311A (A,D) and SAMHD1115-626 D311A/T592D (B,E). (A) dATP concentrations (μM): 0 (red); 10 (orange); 15 (yellow); 20 (green); 30 (blue); 80 (purple). (B) dATP concentrations (μM): 0 (red); 1 (orange); 8 (yellow); 10 (green); 24 (blue); 44 (purple). (D) GTP concentrations (μM): 0 (red); 6 (orange); 10 (yellow); 12 (green); 20 (blue); 50 (purple). (E) GTP concentrations (μM): 0 (red); 8 (orange); 10 (yellow); 15 (green); 25 (blue); 35 (purple). (C,F) Tetramer fraction plotted as a function of nucleotide concentration from multiple sedimentation velocity experiments for D311A SAMHD1115-626 (in blue) and D311A T592D SAMHD1115-626 (in red). The curves show the fit of the data to a simple EC50 equation y = x/(EC50+x).

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