Figure 5 | Scientific Reports

Figure 5

From: Effects of T592 phosphomimetic mutations on tetramer stability and dNTPase activity of SAMHD1 can not explain the retroviral restriction defect

Figure 5

Kinetics of SAMHD1115-626 tetramer association/dissociation.

Fluorescence polarization (left Y axis) measured as a function of time following addition of 0.5 mM dATP to SAMHD1115-626 constructs. (A) Catalytically competent SAMHD1115-626 WT and T592D. (B) Catalytically inactive SAMHD1115-626 D311A and Y146S/Y154S. Kinetics of dATP depletion (right Y axis) was measured for these samples using NMR-based dNTPase assay and plotted as solid black and dashed green lines.

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