Figure 3: Relative expression of genes in cumulus cells.
From: Repeated superovulation may affect mitochondrial functions of cumulus cells in mice
Gene expression was evaluated by qRT-PCR with β-actin used as a house-keeping gene. The relative expression of POLGA is shown in (A) COX1, CYTB, ND2, and ND4 are shown in (B–E), respectively. For each gene, 12 mice were used. *Presents P < 0.05; **presents P < 0.01; R0: natural estrus cycle; R1 (n = 12), one superovulation; R3 (n = 12), three superovulations; R5 (n = 12), five superovulations.
