Figure 1 | Scientific Reports

Figure 1

From: Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes

Figure 1

System schematics.

(a) The optical set-up. The illumination light (shown in green) comes from a collimated laser source and is modified to generate multiple Bessel beams. An array of Bessel beams can be generated either be by an SLM (Right) or by a single axicon that forms one Bessel beam which then can be split using a 1D grating located at the rear pupil of illumination objective lens (Left). Multiple planes (depicted by red, purple, blue and green dashed lines) are illuminated by an array of Bessel beams scanning along × direction. The detection light (shown in red) collected by a microscope system, is modified by MFG grating pattern on another SLM, before it forms an image on CCD. (b) Schematics of multiple Bessel beam illumination. Unlike conventional light sheet illumination (left), the illumination arm (right) has multiple (four is shown here) Bessel beams hitting sample simultaneously.

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