Figure 5
Imaging results of a 3D volume of C. elegans after deconvolution.
PM2 system has two focal planes (z planes) illuminated and detected simultaneously. Two focal plane has separation 200 um. Lateral field of view per focal plane: 340 μm × 340 μm. Objective lens: Olympus 10X/0.25. Sample is scanned along z direction for 10 times, with a scanning step 20 μm. (a) presents 3D information as a stack of focal planes. Different colors represent different focal plane along ‘z’ direction. Top and bottom figures in (a) are two independent measurements at different location of the C. elegans sample. (b,c) A few selected 2D focal planes from (a). Each two focal planes (separation: 200 μm) at same row are simultaneously imaged by a single camera. Sample: randomly distributed C. elegans. Sample density: 16000 animals/100 uL. Scale Bar: 40 μm.
