Figure 2
From: Negative Feedback Regulation of HIV-1 by Gene Editing Strategy
HIV-1 infection stimulates cleavage of integrated viral DNA upon induction of Cas9.
The LTR−80/+66-Cas9 reporter TZM-bl cell line transduced with three different MOI (2, 4 and 8) of lentivirus expressing gRNA A/B (LV-gRNA A/B) or control (empty LV) was infected with HIV-1JRFL or HIV-1SF162 and after 48 hours, cells were harvested and protein expression was determined by Western blot (a), the level of integrated HIV-1 LTR cleavage upon induction of Cas9 after viral infection was detected by PCR/DNA gene analysis (b) and transcriptional activity of the integrated HIV-1 promoter was evaluated by luciferase reporter assay (c).
