Figure 3
From: Microbial Cryptotopes are Prominent Targets of B-cell Immunity
Evaluation of CI against different CMV Ags.
The highly immunogenic CMV Ags glycoprotein B (gB, UL55), pUL32 (pp150) and major structural protein pp65 (UL83) were probed with use of human CMV immune sera (n = 11) under native (Tris-buffer) and denaturing (Urea-buffer) conditions. (A) The difference in signals obtained for each serum sample was expressed as cryptotope index (CI). Bars indicate median CI measured and error bars indicate the interquartile range (IQR). Dashed line indicates median CI of whole CMV (n = 109). (B) Same data set expressed as scatterplot indicating ODTris and ODUrea of each serum for the respective antigen. (C) Western blot imaging of HEK 293 cells transfected with pUL32-, pp65- and gB (gene) and mock-transfected, probed with IVIG.
