Figure 3
From: Modulation of IL-6 induced RANKL expression in arthritic synovium by a transcription factor SOX5
Pro-inflammatory cytokines promote the interaction of SOX5 with RANKL.
(A) immunofluorescence microscopy used to identify co-staining of SOX5 and RANKL in RA synovium. Arrows indicate SOX5 and RANKL double-positive. Scale bar, 100 μm. (B) Confocal microscopy indicated that IL-6 enhances apparent SOX5 co-localization with RANKL in MH7A. Representative laser-scanning confocal micrographs demonstrate the distribution of SOX5 (red), RANKL (green) and overlay (yellow). (C) Quantitative analysis of colocalized area of SOX5 and RANKL by comparing the colocalized area to the total immunofluorescent area. Scale bar, 100 μm. All experiments were performed in triplicate and were repeated three times.
