Figure 4
From: Modulation of IL-6 induced RANKL expression in arthritic synovium by a transcription factor SOX5
RANKL mRNA expression in SOX5 knockdown or overexpression MH7A.
(A) Expression of SOX5 in SOX5-shRNA transfected MH7A. Values are means ± SD. (B) IL-6 induced RANKL expression in SOX5-shRNA treated or mock-transfected MH7A. Values are means ± SD. (C) Expression of SOX5 in MH7A after Ad-SOX5 transfection. (D) RANKL expression in Ad-SOX5 or Ad-EGFP transfected (control) MH7A. Values are means ± SM. Blank in Figure A to D represents non-virus infection. (E) A representative gel of the amplified RANKL DNA immunoprecipitated with SOX5 antibodies in ChIP assay. Rabbit IgG served as negative control. (F) qPCR analyses SOX5 immunoprecipitates for the enrichment of RANKL-specific DNA in IL-6 treated MH7A cells. Fold enrichment of RANKL containing sequences in SOX5 ChIP was determined by comparing enrichment with IgG. All experiments were performed in triplicate and were repeated three times.
