Table 1 The sequence of primers used in the present study for in vitro real-time fluorescence quantitative PCR.
Genes | Primers | Primer Sequence (5′-3′) | Product Length (bp) |
|---|---|---|---|
Runx-2 | Forward | TGCACCTACCAGCCTCACCATAC | 105 |
Reverse | GACAGCGACTTCATTCGACTTCC | ||
Osx | Forward | TATGGCTCGTGGTACAAG | 200 |
Reverse | TCAGATGGGTAAGTAGGC | ||
COL-1 | Forward | GAAGGCTGGAGAGCGAG | 132 |
Reverse | CGGGACCTTGTTCACCTC | ||
Wnt1 | Forward | ATTTTGGTCGCCTCTTTG | 140 |
Reverse | GTGGCATTTGCACTCTTG | ||
Lrp6 | Forward | CAGCACCACAGGCCACCAA | 227 |
Reverse | TCGAGACATTCCTGGAAGAG | ||
β-catenin | Forward | GGAAAGCAAGCTCATCATTCT | 171 |
Reverse | AGTGCCTGCATCCCACCA | ||
β-Actin | Forward | GCCAACACAGTGCTGTCT | 114 |
Reverse | AGGAGCAATGATCTTGATCTT |
