Table 2 The sequence of primers used in the present study for real-time fluorescence quantitative PCR analysis in rabbit bones.
Genes | Primers | Primer Sequence (5′-3′) | Product Length (bp) |
|---|---|---|---|
Runx-2 | Forward | CAGTCTTACCCCTCTTACC | 130 |
Reverse | CATCTTTACCTGAAATGCG | ||
BMP2 | Forward | GGACGACATCCTGAGCGAGT | 117 |
Reverse | CGGCGGTACAAGTCCAGCAT | ||
Osteocalcin | Forward | TTGGTGCACACCTAGCAGAC | 216 |
Reverse | ACCTTATTGCCCTCCTGCTT | ||
Wnt1 | Forward | CTCCACGAACCTGCTAACTG | 226 |
Reverse | GACGATCTTGCCGAAGAGG | ||
Lrp6 | Forward | GCTTGGCACTTGTATGTAAA | 179 |
Reverse | TGGGCTAAGATCATCAGACT | ||
β-catenin | Forward | GACACGGACCACACGCACAA | 173 |
Reverse | CCGAGCAGCAGCAAGTCTTCT | ||
GAPDH | Forward | CATCATCCCTGCCTCCACTG | 183 |
Reverse | GATGCCTGCTTCACCACCTT |
