Figure 4
From: Anisotropic engineered heart tissue made from laser-cut decellularized myocardium
Functional assessment of EHTs.
Measurements of physiological function in laser-cut EHTs. All results were obtained from NRVMs seeded into laser-cut decellularized porcine myocardium. (a) Representative twitch stress records after culture for 9 (blue, n = 11), 16 (red, n = 8) and 23 days (black, n = 12). (b) The effect of culture time and uniaxial stretch on twitch stress. (c) The effect of culture time on the slope of the length-stress relationship in EHTs (increase in mN/mm2 per unit engineering strain ANOVA, Tukey post hoc, *p < 0.05). (d) Representative twitch stress records from 15 day-old EHTs cultured in control media (black) or in media containing thyroid hormone T3 (teal). (e) The effect of T3 treatment (treated n = 10 vs. non-treated n = 12) on peak stress through repeated measurements of EHTs after 5, 8, 12 and 15 days in culture. (f) The effect of T3 treatment on time elapsed between stimulus to peak stress (Time-to-peak, TTP, repeated measures ANOVA,*p < 0.01).
