Figure 4
Phenotypes of GmWRP1-silenced plants.
(A) Expression analysis of GmWRP1 in silenced plants. Expression of GmWRP1 was performed by qRT-PCR using a soybean actin gene as control. The results shown are from five individual empty BPMV vector control plants (BPMV-0) and GmWRP1-silenced plants (BPMV-GmWRP1). The asterisks indicate that the GmWRP1 transcript levels in silenced plants are significantly different from those in control plants (t test, P = 0.01). (B) Early senescence of silenced plant leaves. The pictures of representative control plants inoculated with empty vectors (BPMV-0) and silenced plants inoculated with silencing vectors (BPMV-GmWRP1) were taken 7 weeks after BPMV inoculation. (C) Reduced nodule formation in silenced plants. The pictures of root nodules collected 7 weeks after BPMV inoculation from two representative control plants (BPMV-0) and four silenced plants (BPMV-GmWRP1) are shown. The number of nodules per gram of fresh root weight from at least five plants was also determined and shown above the picture. The asterisks indicate that the root nodule numbers in silenced plants are significantly different from those in control plants (t test, P = 0.01).
