Figure 5
Premature leaf senescence in GmExo70J7 and GmExo70J8-silenced plants.
(A) Expression analysis of GmExo70J7 and GmExo70J8 in silenced plants. Expression of GmExo70J7 and GmExo70J8 was performed by qRT-PCR using a soybean actin gene as control. The results shown are from five individual plants inoculated with the empty BPMV vector (BPMV-0) or a BPMV derivative vector containing a target gene fragment for GmExo70J7 GmExo70J8 (BPMV-target). The asterisks indicate that the GmExo70J7 and GmExo70J8 transcript levels in silenced plants are significantly different from those in control plants (t test, P = 0.01). (B) Early senescence of silenced plant leaves. The pictures of representative control plants inoculated with empty vectors (BPMV-0) and silenced plants inoculated with silencing vectors (GmExo70J7 and GmExo70J8) were taken 7 weeks after BPMV inoculation.
