Figure 2 | Scientific Reports

Figure 2

From: Suppression of c-Kit signaling induces adult neurogenesis in the mouse intestine after myenteric plexus ablation with benzalkonium chloride

Figure 2

Location of NADPH-positive neurons.

Three weeks after BAC treatment, newly appearing neurons in W/Wv mice (n = 3) were identified by NADPH and PGP9.5 staining using cross sections (c,d) and compared with staining in untreated tissue (n = 3) (a,b). (a,b) The myenteric plexus contained NADPH+ cells (a) and PGP9.5+ cells ((b), arrowheads). There were no NADPH+ neuron-like cells or PGP9.5+ cells beyond the myenteric plexus. (c) In samples obtained 3 weeks after BAC treatment, new NADPH+ cells (arrowsheads) were located in the subserosal (SS) and longitudinal muscle (LM) layers, areas where neurons do not normally exist. (d) PGP9.5-positive neurons (arrowheads) located in the subserosal layer. PGP9.5 (red), DAPI (blue), MU, mucosa; CM, circular muscle layer; MP, Myenteric plexus; LM, longitudinal muscle layer; SS, subserosal layer; Scale bar, 100 μm (a,c) or 30 μm (b,d).

Back to article page