Figure 3 | Scientific Reports

Figure 3

From: Suppression of c-Kit signaling induces adult neurogenesis in the mouse intestine after myenteric plexus ablation with benzalkonium chloride

Figure 3

Characteristics of ectopic neurons.

Whole-mount preparations from W/Wv mice 3 weeks after BAC treatment were used to characterize ectopic neurons by immunohistochemistry. (a) Numerous PGP9.5-positive nerve fibers were observed in the LM. Scale bar, 50 μm. (b) PGP9.5-positive cells together with fibers were detected in the SS. Scale bar, 20 μm. (n = 10 for (a,b)). (c) Most ectopically appearing PGP9.5-positive neurons were also BrdU-positive in the SS (n = 5). Scale bar, 50 μm. (d) Calretinin immunoreactive cells and fibers were observed in the SS (n = 5). Scale bar, 20 μm. (e) Some regenerating nerve fibers were TH-positive in the SS (n = 4). Scale bar, 50 μm. (f) GFAP-positive cells and processes were also detected in the SS (n = 7). Scale bar, 50 μm.

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