Figure 4 | Scientific Reports

Figure 4

From: Suppression of c-Kit signaling induces adult neurogenesis in the mouse intestine after myenteric plexus ablation with benzalkonium chloride

Figure 4

Electron microscopy of ectopic ganglia in the SS.

BAC treated tissue from W/Wv mice was weakly stained with NADPH-diaphorase to detect neurons and tissue was then processed for electron microscopy (EM) (n = 3). (a) Low power EM magnification. A typical ganglion containing both neurons (N) and glia (G) in the SS is shown. SS, subserosal layer; LM, longitudinal muscle layer. Scale bar, 5 μm. (b) Enteric neurons in ectopic ganglia had large and few heterochromatin nuclei. Osmium black (double arrowheads) staining in the cytosol indicates NADPH-diaphorase activity. Basal lamina (arrowheads) surrounded the ganglion. Nerve bundles containing synaptic vesicles (arrows) were also observed in the ganglion. Scale bar, 1 μm. (c) Enteric glial cells with heterochromatin-rich nuclei were detected in the ectopic ganglion. The 10-nm filaments in the cytosol were also observed in glial cells (inset, arrows). Scale bar, 2 μm; inset, 500 nm.

Back to article page