Figure 4: Expression analysis of PPP1. | Scientific Reports

Figure 4: Expression analysis of PPP1.

From: RETRACTED ARTICLE: PPP1, a plant-specific regulator of transcription controls Arabidopsis development and PIN expression

Figure 4

(a,b) Root whole-mount in situ RNA hybridization at 3 DAG performed with PPP1-specific antisense (a) and sense (b) probes. (c) PIN2p::GUS expression in the primary root meristem (5 DAG). (d) PPP1p::GUS activity in a flower (32 DAG). (e) Apical portion of wild type seedling (3 DAG) probed with PPP1 antisense transcript (arrowheads indicate leaf primordia). (f–h) PPP1p::GUS activity in a lateral root primordium (10 DAG; f), in the shoot apical meristem region of a seedling (7 DAG; g), in the vasculature of a true leaf (20 DAG; h). (i–k) PPP1:GFP (i), GFP:PPP1 (j) and nuclear-localized JKD:GFP66 (k) in onion epidermis cells (white arrowheads indicate cytoplasmic signals, red signals indicate PI-stained cell walls). l) Root stem cell niche showing 35S::PPP1:GFP expression in nuclei and cytoplasm. (m,n) Details on 35S::PPP1:GFP localization in reporter lines exhibiting strong (m) or weaker reporter expression (n). Green signals indicate reporter protein localization in nucleus and cytoplasm. Bars: a–c, e–g = 50 μm; d,h = 1 mm; i–k = 100 μm; l = 25 μm; m = 10 μm; n = 20 μm.

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